Journals : SERBD-IJMS

Category : Life Sciences
Type : Research Article
Title : In-vitro Studies on Mass Propagation of Bambusa balcooa Roxb.
Submitted By : Shipra Shukla
Corresponding Author : Ankur Bhardwaj
Members :
Member 1 : Ankur Bhardwaj
Member 2 : Shipra Shukla
ABSTRACT :
Bambusa balcooa is a clumping bamboo, ornamental plant of Indian origin. It is popular in Vietnam as food, and can be used as short life temporary constructions and have a variety of usages. The demand of it is increasing enormously and micro propagation promises to cope up with the forecasted shortage. In a short period of time large number of bamboos can be produced in the laboratory via tissue culture method, starting from elite selected genotypes. The treatment of nodal explants with mercuric chloride and sodium hypochlorite for 5 minutes resulted in the highest survival (90%) of explant with minimal microbial infection (5%). Nodal explants were culture on MS medium supplemented with different concentration of BAP and Kinetin. Explants cultured on MS media fortified with BAP (5ppm/lit.) and Kn (4ppm/lit.) showed highest percentage of shoot proliferation after 7 to 10 days. MS medium supplemented with 5 ppm/lit. of BAP and 4 ppm/lit. of Kn was found to be superior to other hormone combinations with regard to proliferation (up to 90%). Amongst different combinations of BAP and IBA used, MS medium supplemented with 2 ppm/lit. BAP and 2 ppm/lit. IBA was found to be the best for root induction in Bambusa balcooa. Visual observations of cultures indicated that higher concentration of BAP (2 ppm/lit.) and IBA (2 ppm/lit.) induces shoot induction. After hardening, rooted plantlets were successfully transferred to the soil in polythene sleeves with over 75% survivability and recorded normal growth and propagation.
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